ARBC2018 at Taipei Zoo, Taiwan

13th Asian Reproductive Biotechnology Congress, 3~6 May 2018

Reproductive biotechnology for defining, delineating and displaying the world of human and animal

ARBC2018_agenda.pdf » PlenarySpeaker.pdf » 3May_Thu_Speaker.pdf » 4May_Fri_Speaker.pdf » 5May_Sat_Speaker.pdf » 6May_Sun_Speaker.pdf »

Plenary Speakers

Time

Speaker

Paper Title

From

3 May (Thu)

11:00~11:30AM

Janine L. Brown

HORMONE MONITORING: AN IMPORTANT TOOL FOR BREEDING MANAGEMENT OF WILDLIFE SPECIES

USA

4 May (Fri)

09:00~09:30AM

Thomas B. Hildebrandt

THE ART OF ART IN WILD PATIENTS

Germany

5 May (Sat)

09:00~09:30AM

Pascal Mermillod

MIMICKING THE MATERNAL ENVIRONMENT TO OPTIMIZE THE PRODUCTION OF EMBRYOS IN VITRO IN WILD AND DOMESTIC MAMMALS

France

6 May (Sun)

09:00~09:30AM

Pierre Comizzoli

GONADAL TISSUE PRESERVATION IN RARE AND ENDANGERED SPECIES

USA

 

Taipei Zoo

Opening Hours:
Open all year (except on Taiwanese New Year's eve) and From 9AM to 5PM (no entry to the zoo after 4PM)
Address: No. 30, Sec. 2, Xinguang Rd., Wenshan Dist., Taipei City 11656, Taiwan, R.O.C.
Tel: +886-2-2938-2300#630 (Visitor Center)
Fax: +886-2-2938-2316

Venue
International Conference Hall
Third Floor, Panda House
Taipei Zoo

Panda House »

Hand-On Training of Flow Cytometric Sperm Assay

Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding.

Assays can be performed on fresh or post thawed diluted semen. Up to 5 000 spermatozoa can be evaluated in seconds.

Sperm Chromatin Structure Assay: Detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm.
Viability: Membrane permeability is an indicator of cellular viability. Through membrane integrity, this test indicates percentage of viable spermatozoa.
Sperm Count: Assess accurate cell numbers and population percentages without reference beads.
Oxidation Level: Measure the intracellular level of Reactive Oxygen Species needed for sperm function but that are harmful at a high level.
Viability and Acrosome Integrity: The acrosome is essential for fertilization. Two of the stains monitor the integrity of the acrosome and of the membrane, simultaneously. The third fluorochrome is sperm-specific and thus allows to remove debris from the analysis.
Mitochondria Activity: Analyze membrane potential (polarized, depolarized) to show the integrity of the mitochondria. To reach the ovocyte, the sperm needs energy which is produced by the mitochondria.
Membrane Fluidity: Membrane fluidity is critical for proper cell function and exchange with the outside environment. This assay assesses phospholipid disorder in the membrane.

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Hand-On Training of Oocyte Freezing

Human
Farm Animal
Zoo Animal
Lab Animal
Reptiles
Amphibia
Fish
Insect

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